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Disposable cups and plastic bags levies proposed

first_imgThe Irish government has proposed new levies on plastic bags, disposable cups and waste disposal.The Republic of Ireland Minister for Communications, Climate Action and Environment Richard Bruton confirmed the measures.He said they were aimed at encouraging more sustainable behaviour and were a key part of the Government’s Climate Action Plan. Under the proposals announced today (Weds), the existing plastic bag levy will increase from the current rate of 22c to 25c.It will also be extended to include medium weight plastic bags, which are often referred to as a ‘bag for life’.A waste recovery levy of €5 a tonne would apply to the three key waste recovery options: landfill, incineration and export.An increase of the landfill levy by €5 per tonne to €80 per tonne has also been proposed. Perhaps, most controversial of all is a new levy on disposable cups.The rate is to be determined following market research, but likely to be 10c, 15c or 25c, according to the department.The new and increased levies will be in place either next year or in 2021.Disposable cups and plastic bags levies proposed was last modified: November 6th, 2019 by Staff WriterShare this:Click to share on Facebook (Opens in new window)Click to share on Twitter (Opens in new window)Click to share on LinkedIn (Opens in new window)Click to share on Reddit (Opens in new window)Click to share on Pocket (Opens in new window)Click to share on Telegram (Opens in new window)Click to share on WhatsApp (Opens in new window)Click to share on Skype (Opens in new window)Click to print (Opens in new window)last_img read more

Dwyane Wade scores 14 in 4th to help Heat stave off Hawks

first_imgTrump campaign, GOP groups attack Google’s new ad policy Wade scored 14 of his 23 points in the fourth quarter and the Heat won their season-high third straight home game by topping the Atlanta Hawks 114-113 on Monday night. It was the highest-scoring quarter of the season for Wade, and it helped Miami beat Atlanta for the first time in four tries.“That’s textbook, Hall of Fame poise,” Heat coach Erik Spoelstra said. “He’s playing his best basketball of the season right now.”FEATURED STORIESSPORTSPrivate companies step in to help SEA Games hostingSPORTSUrgent reply from Philippine ‍football chiefSPORTSPalace wants Cayetano’s PHISGOC Foundation probed over corruption chargesJosh Richardson scored 19 points and Justise Winslow added 18 for the Heat, who pulled into a tie with Charlotte for ninth place in the Eastern Conference. Dion Waiters scored 14 and Kelly Olynyk added 12.Vince Carter scored 21 points, all on 3-pointers, for the Hawks — in his final head-to-head duel with Wade, and tipped his cap afterward. MOST READ Winslow fouled Young with 2:04 left, then picked up a technical as well. Young missed the technical free throw, then made both for the personal to get the Hawks to 111-107. And down by three, after a Heat turnover, Young had a blow-by for a layup that cut Miami’s lead to 112-111 with 1:02 left.Wade got free for a layup on the next possession to put Miami back up by three. Bazemore made a pair of foul shots with nine seconds left, but the Heat inbounded the ball and wound up running out the clock — with the Hawks never catching anyone to foul.“We just fell asleep at the end,” coach Lloyd Pierce said.Wade’s block of Atlanta’s B.J. Johnson in the second quarter was a record-setter of sorts. It was the 1,052nd blocked shot of Wade’s career, including playoffs — the most ever by a guard, one more than Michael Jordan. The regular-season mark still belongs to Jordan, who has 893 to Wade’s 877.“Either way, it’s pretty cool,” Wade said.TIP-INSHawks: Bazemore took six free throws in 23 seconds during the third quarter, after getting fouled on 3-point attempts on consecutive possessions. He went 4 for 6, missing the middle free throw each time. … Young is the 32nd rookie (and first for the Hawks) to have 500 assists in a season. He entered Monday with 499. … John Collins, a South Florida native, missed the game with flu-like symptoms.Heat: Richardson, the biggest soccer fan in the Heat locker room, did some futbol-style juggling of the basketball during a fourth-quarter stoppage. Then he hit a 3 for a 104-95 lead. … Hassan Whiteside (hip strain) returned after missing three games. He played as a reserve for the first time since April 8, 2016, ending a streak of 202 consecutive games played as a starter (including postseason). … James Johnson (shoulder) was again active, but missed his sixth straight game. .. Goran Dragic (left calf strain) missed his second consecutive game.MOVING UPCarter passed Reggie Miller on the career scoring list — doing so, fittingly, on a 3-pointer. Carter’s third 3 of the first half gave him 25,280 points, one more than Miller for 20th place. Carter and Miller are both among the top seven 3-point shooters in NBA history. P2.5 B shabu seized in Makati sting, Chinese national nabbed PH underwater hockey team aims to make waves in SEA Games PLAY LIST 02:42PH underwater hockey team aims to make waves in SEA Games01:44Philippines marks anniversary of massacre with calls for justice01:19Fire erupts in Barangay Tatalon in Quezon City01:07Trump talks impeachment while meeting NCAA athletes02:49World-class track facilities installed at NCC for SEA Games02:11Trump awards medals to Jon Voight, Alison Krauss P2.5 B shabu seized in Makati sting, Chinese national nabbed LATEST STORIES 1 dead, 3 injured in Quezon road crash Miami Heat guard Dwyane Wade (3) goes up to shoot against Atlanta Hawks forward DeAndre’ Bembry (95) during the second half of an NBA basketball game, Monday, March 4, 2019, in Miami. (AP Photo/Wilfredo Lee)MIAMI — Dwyane Wade got into the record book for defense.And he saved the Miami Heat with offense.ADVERTISEMENT WADE’S BLOCKSJohnson became the 449th player to have a shot blocked by Wade. Joe Johnson and Ben Gordon each got blocked 12 times by Wade, more than any other player. The tallest players who Wade got were both listed at 7-foot-3 — former Heat center Zydrunas Ilgauskas, and Dallas’ Kristaps Porzingis. The shortest to have one swatted by Wade was Earl Boykins, listed at 5-foot-5.UP NEXTHawks: Host San Antonio on Monday. It’ll be the first Hawks-Spurs game of the season.Heat: Visit Charlotte on Wednesday. The Heat are 0-2 this season against the Hornets.Sports Related Videospowered by AdSparcRead Next Hong Kong tunnel reopens, campus siege nears end “He’s been a very dominant player for a long time and on your way out it’s easy to say, ‘I’m going to ride this out and enjoy it,’” Carter said. “But he’s doing it like most Hall of Famers would do it — go at it and leave my greatness on the floor every night until you don’t see it anymore.”Kent Bazemore had 18 points and Trae Young finished with 14 points and eight assists for the Hawks. Atlanta also got 14 points from Dewayne Dedmon and 13 from Kevin Huerter.Wade made three consecutive jumpers, including a 3, to spark a 12-2 run in the fourth that took the Heat from down one to up by nine with about five minutes left.“Got a couple shots to fall, you get in a rhythm,” Wade said. “We needed it. Obviously, we’ve had a lot of trouble with this team.”The end was frantic, starting when Carter hit a pair of late 3-pointers to start the Atlanta rally.ADVERTISEMENT Private companies step in to help SEA Games hosting Raptors and Rockets to play 2 preseason games in Japan Lacson backs proposal to elect president and vice president in tandem SEA Games hosting troubles anger Duterte Don’t miss out on the latest news and information. View commentslast_img read more

KURT RUSSELL IS FILMING A SANTA MOVIE IN TORONTO

first_img Twitter KURT RUSSELL IS FILMING A SANTA MOVIE IN TORONTO RIGHT NOWKurt Russell, 1980s hot guy turned legendary Hollywood actor, has been spotted around Toronto lately rocking a long white beard and sometimes a red velour twinset.The Tombstone, Hateful Eight and Escape From New York star is reportedly in town to film a Netflix original Christmas movie produced by Chris Columbus – the guy behind Home Alone.  READ MORE T’isn’t the season! Kurt Russell, 66, looks just like St Nick as he gets to work in Toronto filming a Christmas movieChristmas might be the furthest thing from most people’s minds right now. But not for Kurt Russell, who was dressed as Santa Claus in the middle of Toronto on Thursday. The 66-year-old was filming an upcoming Xmas movie for Netflix, and made a pretty excellent St Nick. READ MOREKurt Russell to Play Santa Claus in Netflix Christmas MovieJudah Lewis, the star of Netflix’s ‘The Babysitter,’ and ‘Big Little Lies’ actress Darby Camp are also starring in the fresh take on the holiday family film.Kurt Russell is bringing Christmas cheer to Netflix.The veteran actor is teaming with Judah Lewis, the star of Netflix’s horror-comedy film The Babysitter, along with Darby Camp of Big Little Lies, to star in an untitled Christmas movie for the streaming giant. READ MORE Advertisement Facebookcenter_img Advertisement Login/Register With: Advertisement LEAVE A REPLY Cancel replyLog in to leave a comment last_img read more

Hurricane Irma packing 175mph winds

first_img Related Items:#HurricaneIrma, #magneticmedianews Facebook Twitter Google+LinkedInPinterestWhatsApp Turks and Caicos, September 5th, 2017 – Providenciales – Hurricane Irma has not even struck any land mass as yet, but she is striking up a whole lot of fear as the storm continues to grow in intensity and makes it way toward so many islands of the Caribbean.  At present, Irma’s wind speeds are up to 175 mph and her trajectory, according to National Hurricane Center model, will send her across Anguilla, British Virgin Islands, Antigua and Barbuda, St Kitts and Nevis, Puerto Rico, Dominican Republic, Haiti, the Turks and Caicos Islands, Inagua and Ragged Island in The Bahamas, along Cuba and the Florida Keys. However the path of Irma has had variations and so forecasters say we have to wait to see what Irma will do once the hurricane interacts with land.  All officials, from every country we have checked in with, are advising residents to take this storm seriously and to be prepared.   By Deandrea Hamilton Facebook Twitter Google+LinkedInPinterestWhatsApp New, stringent posture on illegal construction makes fines, personal demolition and possible deportation legal says PDM Minister, law now passed August 30th – One Year since Hurricane Irma named FortisTCI announces bid to hike electricity bills, cites record $42m response to hurricanes as destabilizing Recommended for youlast_img read more

Twitter Rolling Out Analytics Tool

first_imgIn the last five years, Twitter has grown to 100 million monthly users, says the company, with about half of them logging in at least once a day. The analytics tool was debuted at the TechCrunch Disrupt event where Twitter’s director of Web business development April Underwood said the platform is driving 100 million clicks per day to sites across the Web, reports TechCrunch’s Leena Rao.Underwood told attendees that the analytics tool will be free. The company also plans to make the Twitter Web Analytics API available to developers to incorporate it into their products.TechCrunch has video from Underwood’s announcement at the Disrupt show. On Tuesday Twitter announced that it’s launching a new analytics tool that will initially be available to a select group of partners as a pilot program, but will be made available to everyone in the “next few weeks.”The tool will enable Web site developers to more accurately measure Twitter traffic referrals and how well the Twitter features they’ve integrated into their sites are performing.The tool is essentially the result of Twitter’s acquisition of BackType in July. According to the company’s announcement yesterday, the tool will have three core functions: the ability to understand how content is being shared across Twitter; the ability to measure the traffic Twitter is sending to a site; and measuring the effectiveness of the Tweet button integration.last_img read more

Wilmington Middle School Drama Club Presents Seussical The Musical On March 2123

first_imgWILMINGTON, MA — The Wilmington Middle School Drama Club presents its spring musical, “Seussicial The Musical” on Thursday, March 21, 2019 (7pm); Friday, March 22, 2019 (7pm); and Saturday, March 23, 2019 (1pm) in the Wilmington Middle School Auditorium.Come enjoy the story of Horton and some of our favorite Dr. Seuss characters. Tickets are available at the door. Tickets cost $10 for adults and $7 for students (12 and under).Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email wilmingtonapple@gmail.com.Share this:TwitterFacebookLike this:Like Loading… RelatedWilmington Middle School Drama Club Presents ‘Alice In Wonderland’ On March 22-24In “Education”5 Things To Do In Wilmington On Thursday, March 21, 2019In “5 Things To Do Today”5 Things To Do In Wilmington On Friday, March 22, 2019In “5 Things To Do Today”last_img read more

Karma will reveal the secondgeneration Revero in Shanghai

first_img 2019 Honda Odyssey offers plenty of room and features for families 2019 Jeep Cherokee can handle the rough stuff 12 Photos 0 52 Photos Honda OdysseyThe feature-rich Honda Odyssey minivan also retains its number-two slot from last year’s list. While the 2019 model doesn’t see any changes from last, it’s still a darn good choice for families on the go with reconfigurable seats, Wi-Fi and an excellent rear-seat entertainment system. The Cabin Watch video system lets parents keep an eye on their little darlings without turning around in their seats, while Cabin Talk amplifies their voice so no yelling is required.The Odyssey is powered by a 3.5-liter V6 rated for 280 horsepower and 262 pound-feet of torque. Most trims get a nine-speed automatic but the top Touring and Elite trims now use a 10-speed automatic with stop-start technology. The Honda Odyssey is made in Lincoln, Alabama. 2019 Chevy Colorado ZR2 Bison: An off-road animal Honda PilotDropping down to seventh place for 2019 is the Honda Pilot. Like its two-row Passport sibling, the three-row Pilot offers parent-focused technology like Cabin Talk as well as myriad standard driver-assistance features through the Honda Sensing tech suite. Honda’s largest crossover offers up nearly 84 cubic feet of cargo space. Honda’s 3.5-liter V6 engine shows up again, making a serviceable 280 horsepower and 262 pound-feet of torque. Touring and Elite trims get a nine-speed automatic transmission while lower trims have to make do with a six-speed auto. The Honda Pilot is made in Lincoln, Alabama. 18 Photos Shanghai Motor Show 2019 Tags 10 Photos 71 Photos 2017 Karma Revero looks like a Fisker, but much improved underneath 49 Photos Share your voice Jeep CherokeeKeeping its top-of-the-list placement, the Jeep Cherokee is 2019’s most American-made car. Refreshed for 2019, the compact crossover now features more tech, better cargo space and a slightly tweaked look. It’s available in no fewer the nine trims, including the off-road specific Trailhawk and a fancy-pants Trailhawk Elite.For 2019, the Cherokee gets a new 2.0-liter, turbocharged, four-cylinder engine with 270 horsepower and 295 pound-feet of torque, though naturally aspirated I4 and V6 choices are also available. Regardless of engine, a nine-speed automatic transmission gets the power to the pavement — or dirt as the case may be. The Cherokee is made in Belvidere, Illinois. Post a comment 2019 Honda Pilot Elite: A smoother, tech-rich crossover SUV 2019 Honda Ridgeline: The commuter’s pickup truck Chevrolet ColoradoMaking its debut in the top 10 is the Chevrolet Colorado. Shown here in the tough ZR2 Bison off-road spec, the Chevy Colorado is an excellent truck and one of the two midsize pickups you can get with a diesel engine. Adding to the ZR2’s two-inch lift and front and rear locking differentials, the Bison gets beefier skid plates (trust me, that’s a good thing), steel bumpers and integrated recovery points. Heck, you can even get a snorkel.Base models get a 2.5-liter four-cylinder engine with a six-speed manual transmission, but a more popular choice is the 3.6-liter V6 gas engine with 308 horsepower and 275 pound-feet of torque, mated to an eight-speed automatic transmission. Meanwhile, the 2.8-liter diesel pumps out 186 horsepower and a delicious 369 pound-feet of twist and is mated to a six-speed automatic. The Chevy Colorado is built in Wentzville, Missouri. 2019 GMC Sierra Denali review: So close to greatness 2019 Honda Passport: A well-rounded midsize offering More From Roadshow 68 Photos 2019 Chevrolet Corvette Grand Sport loves to hustle 69 Photos Share your voice 2019 Acura MDX adds new features and an A-Spec model Acura RDXThe third-generation Acura RDX squeaks into the top ten list with larger proportions, plenty of interior space and better handling than its predecessor. Forward collision warning with collision mitigation braking, adaptive cruise control that works even in low-speed traffic, lane-keeping steering assist and road departure mitigation are all standard across the board. Like its MDX counterpart, the RDX is available with the A-Spec styling package.The RDX sports a 2.0-liter, turbocharged, four-cylinder engine. Output is stated at 273 horsepower and 280 pound-feet of torque, and it’s mated to a 10-speed automatic transmission. The new RDX is built in East Liberty, Ohio. 2019 GMC Canyon: A fancier Colorado Our first look at the “all-new” Revero. Karma Automotive Karma Automotive might have been quiet for some time, but at the upcoming Shanghai Motor Show, the California-based automaker will have plenty to talk about. Karma on Thursday revealed a teaser photo showing the 2020 Revero, the second generation of the car that was derived from the Fisker Karma. The car debuts at Auto Shanghai in early April.The 2020 Karma Revero will apparently be “faster, smarter” than the outgoing Revero, and the company also promises it will be “even more stunning in design.” It’s not clear from the shadowy teaser photo exactly how the car’s design will change, but we’re eager to see the apparently “all-new” model. Interested parties will be able to get behind the wheel soon, too, as Karma says the 2020 Revero will be offered to buy or lease in the second half of this year.Powertrain and other mechanical specs for the new car remain a mystery. The existing, first-generation Karma Revero is a plug-in hybrid that Karma says has 50 miles of all-electric range, 403 horsepower and 981 pound-feet of torque.The introduction of the new Revero is the final pillar in what Karma is calling its “Shanghai Big Three.” The company will also use the Chinese show to reveal an all-electric concept car called the Vision, as well as a concept designed with famed design house Pininfarina.Today’s teaser comes with the news that Karma has also invested $7.5 million to upgrade its powertrain research and development facilities at its headquarters in Irvine, California. New tech, including test cells and dynamometers for both gas engines and electric motors, will allow Karma to further its development of “electric motors, generators and range-extended powertrains” in-house, instead of contracting that work to outside companies. It’s a signal that Karma is serious about developing more new models going forward. 26 Photos 3 Chevrolet CorvetteMoving up from the 10th-place slot last year is the 2019 Chevrolet Corvette. While we’ve all been ogling the new mid-engine Corvette, it’s easy to forget what a performance bargain the current-generation ‘Vette is. From the base trim to the Grand Sport to the bonkers ZR1, the Corvette is an American icon.My preference is for the Grand Sport, which strikes a perfect balance between everyday drivability and canyon-carving performance. The 6.2-liter V8 puts out 460 horsepower and 465 pound-feet of torque. You can get it with a seven-speed manual or eight-speed automatic transmission, but the kicker is the borrowed chassis and aero parts from the beefier Z06. Like I said, perfect balance. The Corvette is made in Bowling Green, Kentucky. Acura MDXMoving up one slot to sixth place on the American Made Index is the non-hybrid variant of the Acura MDX. With its SH-AWD system, the MDX is one of the better handling midsize luxury crossovers, and for 2019 the company gives us the A-Spec treatment with a new front fascia and side skirts, 20-inch wheels, wider exhaust tips, unique gauges, a new steering wheel, carbon fiber trim and various Alcantara interior touches.However, both the standard and A-Spec models get a 3.5-liter V6 engine, rated for 290 horsepower and 267 pound-feet of torque going through a nine-speed automatic transmission. The Acura MDX is made in East Liberty, Ohio.  2020 BMW M340i review: A dash of M makes everything better 81 Photos 48 Photos 2019 Honda Ridgeline review: Light duty, heavy punch More From Roadshow Car Industry Comments Hybrids Luxury cars Sedans,Enlarge ImageIt’s all in a day’s work for the Jeep Cherokee, the most American-made car in 2019. Jeep With high trade tensions looming and increasing talk of tariffs, more and more consumers are looking to buy American. Cars.com has released its annual American Made Index, showcasing companies that use the most American-sourced parts and labor in their vehicles. The results may surprise you.Cars.com analyzed more than 100 US-built vehicles for five key data points: manufacturing location, parts sourcing, US employment, engine sourcing and transmission sourcing. You might think something like the Ford F-150 would top the list, but it drops from its No. 5 spot for 2018 and out of the top 10 completely. Even the first-place holder, the Jeep Cherokee, is a pretty global product. It’s a Jeep, sure, but that brand is part of Fiat Chrysler Automobiles, an Italian-owned business.Cars.com surveyed 1,000 people and found that half are concerned about automotive import tariffs, with 41% saying they are unsure if tariffs would make them more likely to buy American. Either way, we’re big fans of all the vehicles on this list. These days, buying American doesn’t necessarily mean sticking with a US-based automaker. Honda PassportThe Honda Passport is a new (well, reborn) entry to both the Honda lineup and the American Made Index. This midsize crossover comes to the fray with great driving dynamics and plenty of tech like the standard Honda Sensing suite of active safety features. An available 8-inch touchscreen gets Apple CarPlay and Android Auto as well as a Wi-Fi hotspot.Under the hood is the stalwart 3.5-liter V6 producing 280 horsepower and 262 pound-feet of torque. However unlike the Ridgeline, the Passport sends that power through a nine-speed automatic transmission. Having said that, just like the Ridgeline, this is the only powertrain available. The Honda Passport is built in Lincoln, Alabama. Honda RidgelineAgain keeping the status quo, the Honda Ridgeline maintains third place on the American Made Index. This crossover-that-looks-like-a-truck provides a better ride than a traditional pickup and gets an awesome lockable trunk right in the floor of the bed. And it doesn’t do the truck stuff too badly, either, as it’s able to carry 1,860 pounds of payload and tow 3,500 pounds.The Ridgeline is powered by a 3.5-liter V6 good for 280 horsepower and 262 pound-feet of torque. That is channeled through a six-speed automatic transmission with available all-wheel drive. It’s built in Lincoln, Alabama. 2020 Hyundai Palisade review: Posh enough to make Genesis jealous 2019 Acura RDX A-Spec: Sharp handling, sharper looks General Motors Chevrolet Honda Jeep Acura 2019 Chevrolet Colorado ZR2 Bison: A tougher off-roader GMC CanyonIf you’re looking for a slightly fancier version of the Colorado that’s more focused on luxe than dirt, check out the GMC Canyon. The top Denali trim gets standard heated and ventilated front seats as well as a heated steering wheel. Maximum payload capability is 1,665 pounds while max towing is a fairly healthy 7,600 pounds.The Canyon is available with the same 2.5-liter four-cylinder engine, 3.6-liter V6 or 2.8-liter diesel as the Chevrolet Colorado. It’s also built in Wentzville, Missouri. Tags 2020 Kia Telluride review: Kia’s new SUV has big style and bigger valuelast_img read more

Vote to end misrule of current govt Moudud

first_imgBNP standing committee member Moudud Ahmed addresses a discussion at the National Press Club on Friday. Photo: Abdus SalamBNP senior leader Moudud Ahmed on Friday urged the voters to get united and cast their votes on 30 December under any situation to free Khaleda Zia from jail and get rid of the “misrule and oppression of the current government”, reports UNB.”I would like to tell the voters that this is your time. You take oath that you’ll cast your votes. This is a struggle of the country’s 100 million voters, not only of the BNP and Jatiya Oikya Front,” he said.Speaking at a discussion, he further said “I call upon all to convince voters that this is the last chance to free Khaleda Zia and get rid of the repression of the current government.”Adarsha Nagarik Andalan and Jatiya Nagarik Andalan jointly organised the programme at National Press Club.Moudud, a BNP standing committee member, said a mass wave has been created in favour of the ‘Sheaf of Paddy’, the electoral symbol of the BNP, across the country and the ruling party will not be able to tackle it, if people can cast their votes.”That’s why we should persuade the voters that they must go to polling stations braving all obstacles, threats and intimidations. We must put up a strong resistance if they (AL) create any obstacles. We have no other alternative to it,” he added.He also asked the BNP leaders and activists not to get frustrated but to make their all-out efforts to get success in the struggle of vote by mobilising people.”I also call upon all to keep in touch with people and organise them to win the election. We have no chance to lose this time as people are with us,” the BNP leader said.He said people are ready to give a befitting reply to the ‘misdeeds’ of the current government through ballots.last_img read more

CLIP170 microtuble found to bind tightly to formins to accelerate actin filament

first_img(Phys.org)—A team of researchers at Brandeis University in Massachusetts, has found that the CLIP-170 microtuble found in cells, which had been known to be important in cytoskeleton development, binds tightly to formins to accelerate actin filament elongation. In their paper published in the journal Science, the team describes their experiments with adding a fluorescent protein to the microtubule to better understand the roll CLIP-170 plays in actin flament assembly. In a Perspective piece on the work done by the team, Klemens Rottner with Universität Braunschweig in Germany also explains the role played by microtubules and actin filaments in the development of the eukaryotic cytoskeleton (the material that holds the shape of cells.) Explore further More information: J. L. Henty-Ridilla et al. Accelerated actin filament polymerization from microtubule plus ends, Science (2016). DOI: 10.1126/science.aaf1709AbstractMicrotubules (MTs) govern actin network remodeling in a wide range of biological processes, yet the mechanisms underlying this cytoskeletal cross-talk have remained obscure. We used single-molecule fluorescence microscopy to show that the MT plus-end–associated protein CLIP-170 binds tightly to formins to accelerate actin filament elongation. Furthermore, we observed mDia1 dimers and CLIP-170 dimers cotracking growing filament ends for several minutes. CLIP-170–mDia1 complexes promoted actin polymerization ~18 times faster than free–barbed-end growth while simultaneously enhancing protection from capping proteins. We used a MT-actin dynamics co-reconstitution system to observe CLIP-170–mDia1 complexes being recruited to growing MT ends by EB1. The complexes triggered rapid growth of actin filaments that remained attached to the MT surface. These activities of CLIP-170 were required in primary neurons for normal dendritic morphology. Thus, our results reveal a cellular mechanism whereby growing MT plus ends direct rapid actin assembly. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Scientists reveal mechanism for cellular remodeling Citation: CLIP-170 microtuble found to bind tightly to formins to accelerate actin filament elongation (2016, May 20) retrieved 18 August 2019 from https://phys.org/news/2016-05-clip-microtuble-tightly-formins-actin.htmlcenter_img Journal information: Science © 2016 Phys.org Components of the eukaryotic cytoskeleton. Actin filaments are shown in red, microtubules are in green, and the nuclei are in blue. Credit: NIH/Public Domain In order for a cell to hold its shape, filaments must grow to the size needed to provide support, similar in some respects to the cartilage found in the nose and other parts of the body. But, in order for the cell to grow to the proper size and shape it needs to be to hold all its components, some type of mechanism must be in place to regulate the growth. Prior research has shown that microtubules serve that purpose, but how the process works, has remained somewhat murky. In this new effort, the researchers found that the CLIP-170 microtuble actually binds very tightly to a protein known as formin, and in so doing is able to play a role in determining how long an actin filment will grow—or more specifically, to actually accelerate elongation.To learn more about the role that CLIP-170 plays in cytoskeleton development, the researchers applied a fluorescent protein to some of them in their lab, and then watched what happened using single cell microscopy during cell maturation. In addition to accelerating actin filament elongation, the team observed it along with mDia1 dimers, tracking filament ends and promoting polymerization (causing it to grow approximately 18 times faster than growth without it), which provided a protective cap. They also found that using another technique allowed them to watch as the mDia1/CLIP-10 complexes were coaxed into action by microtubule ends, which resulted in rapid filament growth. They note that such filament growth is most particularly necessary for dendrite growth in neurons.last_img read more